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KMID : 0381920110410020109
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Korean Journal of Microscopy
2011 Volume.41 No. 2 p.109 ~ p.116
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Expression of NGF in Estradiol Valerate-Induced Polycystic Ovary and CHO Cells
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Choi Baik-Dong
Jeong Soon-Jeong
Jeong Moon-Jin
Lim Do-Seon
Lee Soo-Han
Kim Seung-Hyun
Ko A-Ra
Kim Se-Eun
Kang Seong-Soo
Bae Chun-Sik
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Abstract
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Polycystic ovary syndrome (PCOS) is hormonal imbalance condition as the endocrine and metabolic disorder that nduces the infertility and various complications in reproductive age women. Estradiol valerate (EV) is used hormone replacement therapy in menopausal women and is reported that excessive administration of EV induces the PCOS. Nerve growth factor (NGF) is the factor to regulate the survival and maturation of developing neuronal cell and is also synthesized in ovary. And NGF is overexpressed in EV-induced polycystic ovary (PCO) as previously reported. Therefore, this study examined the possibility of NGF as can be used the biological marker in diagnosis of PCOS, the hormonal imbalance condition, using PCO and CHO (chinese hamster ovarian) cell lines. The concentration of EV treatment is optimized a 1 mg as not influence on the proliferation of CHO cell but 2 mg and 3 mg of EV treatment have the inhibition effect at initial stage. The morphological change was not observed in CHO cell after dose dependent manner treatment of EV. Expression of NGF mRNA and protein is significantly increased at 30 min after EV treatment in CHO cells compared to that of control. And NGF protein expression is strongly increased in PCO tissue, which observed many follicular cysts compared to normal ovary tissue. Taken together, overexpression of NGF may be act as a molecule to induce an abnormal development of follicle, suggesting that NGF can be used as a biological marker in diagnosis of PCOS.
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KEYWORD
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CHO cell, Estradiol Valerate, NGF, PCO, Rat
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